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We previously reported that an increase of column chromatographically measured hemoglobin AI (HbAI) levels in chronic renal failure (CRF) was caused by an increase of the cyanate-hemoglobin adducts (carbamylated hemoglobin). In the present study, we investigated the effects of CRF on Hb Ai levels measured by a spectrophotometric method using phytic acid. Phytic acid, one of the 2, 3-DPG analogs, is known to bind to hemoglobin at its 2, 3-DPG pocket, and therefore the binding of phytic acid on glycosylated hemoglobin is sterically inhibited.<BR>Hb Ai levels were measured in 20 CRF patients without diabetes, whose serum creatinine levels were above 1.5mg/d<I>l</I>. The HbAi values were correlated significantly with BUN (r=0.68, p<0.001, Y=0.04X+4.37, X=BUNmg/d<I>l</I>, Y=-Hb AI%), but did not with FBS (r=0.30, N. S.).<BR>Red blood cells, drawn from a normal subject, were incubated for 3 days in media containing 100, 500 and 1000 mg/d<I>l</I> of urea N, and the HbAI levels were determined by the phytic acid-spectrophotometric method. The concentrations of cyanate, which is in equilibrium with urea in aqueous solution, were 1.25, 3.44 and 7.25 mg/d<I>l</I>, respectively. The Hb Ai levels were elevated with increasing concentrations of cyanate, and the HbAI levels in each medium were 5.7±1.0, 33.7±2.8 and 55.8±3.8%(M±SD, N=4), respectively.<BR>These results suggest that the increase of HbAi levels measured by the phytic acid-spectrophotometric method in CRF can be attributed to an increase of carbamylation, but not glycosylation of hemoglobin, and that cyanate binds to hemoglobin at its 2, 3-DPG pocket.
- 一般社団法人 日本糖尿病学会の論文
一般社団法人 日本糖尿病学会 | 論文
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