Usefulness of Immunological Detection of Both Toxin A and Toxin B in Stool Samples for Rapid Diagnosis of Clostridium difficile-associated Diarrhea
スポンサーリンク
概要
- 論文の詳細を見る
Toxin detection from stool specimens is critical for the diagnosis of <I>Clostridium difficile</I>-associated diarrhea (CDAD). In Japan, only two toxin detection kits targeting toxin A alone are commercially available. We evaluated ImmunoCard Toxin A & B (ImmunoCard), based on enzyme immunoassay for the rapid detection of both <I>C. difficile</I> toxins A and B in stool specimens, compared to a toxin A detection kit (Uniquick) and cytotoxin assay. <I>C. difficile</I> was also cultured from stool specimens and the toxin production type of <I>C. difficile</I> isolates was identified by PCR analysis. Compared to cytotoxin assay, ImmunoCard sensitivity was 86.2%, specificity 93.8%, positive predictive value 91.8%, and negative predictive value 89.4% (n=146). Sensitivity was significantly higher than that of Uniquick (60.0%, p=0.0016). ImmunoCard detected 90.6% of cytotoxin positive specimens with isolated toxin A-positive, toxin B-positive <I>C. difficile</I> strains (Uniquick; 67.9%, p=0.008) and 70.0% of these with isolated toxin A-negative, toxin B-positive <I>C. difficile</I> strains. Although ImmunoCard was slightly less sensitive than cytotoxin assay, it requires no special equipment and completes the entire test in up to 20min. ImmunoCard thus appears very useful in the rapid diagnosis of CDAD in the clinical laboratory. Kits for the detection of both <I>C. difficile</I> toxins A and B should be immediately introduced into Japan to ensure the correct diagnosis of CDAD and infection control.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
- Simple Method for Typing Human Immunodeficiency Virus Type 1 (B, E) by PCR and Chronological Change of Distribution ofSubtype (B to E) in Japan.
- Kinetic Analysis of Anti-HIV Titer in Early Stage of HIV Infection. A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Positive Cases.:A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Posit
- HIV-1 (Human Immunodeficiency Virus Type 1) Mutant Strain Associated with Zidovudine Resistance Detected from a Japanese Patient with Primary HIV-1 Infection.
- Epidemiological and Bacteriological Study on Gonococcal Infections.
- A Case of Trichosporon beigelii Peritonitis in CAPD.