A New Genetic Assay for Rifampicin Susceptibility of Mycobacterium tuberculosis.
スポンサーリンク
概要
- 論文の詳細を見る
A new method for testing rifampicin (RFP) susceptibility of <I>Mycobacterium tuberculosis</I> was developed. This method is based on detection of the internal sequence derived from 71-kDa heat shock protein mRNA in tubercle bacilli heat-treated in the presence of RFP. The target sequence was amplified by reverse transcription and PCR, followed by agarose gel electrophoretic analysis. No amplification occurred in one RFP-susceptible strain by exposure to 45°C for 45 min in Middlebrook 7H9 broth containng RFP (10μg/ml) after overnight incubation at 37°C. On the other hand, an amplified 275-bp product was obtained from the RFP-resistant strain MY-129. In a subsequent trial using 65 clinical isolates, this method defined their RFP susceptibility levels as well as the verification of the MICs obtained by the conventional agar dilution method, with the exception of one RFP susceptible strain. Thus, this method provides a rapid and practical system to determine RFP susceptibility in <I>M. tuberculosis</I>.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
- Simple Method for Typing Human Immunodeficiency Virus Type 1 (B, E) by PCR and Chronological Change of Distribution ofSubtype (B to E) in Japan.
- Kinetic Analysis of Anti-HIV Titer in Early Stage of HIV Infection. A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Positive Cases.:A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Posit
- HIV-1 (Human Immunodeficiency Virus Type 1) Mutant Strain Associated with Zidovudine Resistance Detected from a Japanese Patient with Primary HIV-1 Infection.
- Epidemiological and Bacteriological Study on Gonococcal Infections.
- A Case of Trichosporon beigelii Peritonitis in CAPD.