Enzymic Hydrolysis of Osseomucoid:1. Effect of Protease Examined by "Ferrante Method"

概要

論文の詳細を見る
When the acid polysaccharide precipitates with Fe…, coexisting protein such as serum albumin, if there is any, co-precipitates with chondroitin sulfuric acid. In this case, a small amount of Fe combines also with the protein moiety. It seems probable that the precipitate produced by the combination of osseomucoid and Fe… also containes the neutral glycoprotein together with the acid polysaccharide. Mucoid was precipitated by the addition of Fe… after the hydrolysis by protease. The determination of N and Fe… in this precipitate has given a marked decrease of the above two components within 20 minutes from the beginning of the hydrolysis, and the rate of decrease has shown a slight difference between N and If the part of acid polysaccharide combines, as we suppose, much Fe… while protein holds a little, Fe… in the precipitate could not decrease so much by the hydrolysis of protein, though the amount of N in the precipitate may decrease as the hydrolysis proceeds. The above experimental result, contrary to our expectation, is of fairly interest suggesting a possibility of another conception.
口腔病学会の論文