:Studies on crossed immunoelectrophoresis using anti-plasminogen antibody and antibodies to inhibitors

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Activation of intrinsic pathway of fibrinolysis and its inhibitory system were studied immunologically. Using anti-plasminogen antibody crossed immunoelectrophoresis (CIE) was done in normal plasma on condition that prekallikrein was activated with prekallikrein activator (Pkk. A.; Kabi) which is a mixture of ellagic acid, cephalin, and plasma fraction containing Hageman factor and high molecular weight kininogen. CIE of normal plasma activated with Pkk. A. revealed two peaks, while that of untreated plasma did single peak. It was supposed that newly appeared first peak was plasmin generated by intrinsic pathway and second peak was original plasminogen.Plasma in a case of hereditary angioneurotic edema (HANE) showed a higher and wider first peak in pattern of CIE on the same condition as normal plasma. On the other hand these findings were not observed, when normal plasma was treated with other contact activating agents.CIE using anti-C1-esterase inhibitor (C1-INH) antibody and anti-α2-plasmin inhibitor (α2-PI) antibody were also done in normal plasma. A distoi-tion of ascending shoulder of original peak was seen in both CIE patterns after the activation with Pkk. A.. It seems likely that complexes between kallikrein and C1-INH or α2-PI were generated while activation of prekallikrein.
一般社団法人日本血栓止血学会の論文