Adrenarche in Childhood: I. Development of a New Enzyme-linked Immunosorbent Assay (ELISA) for Dehydroepiandrosterone Sulfate (DHEAS) and Determination of Serum DHEAS Levels in Normal Children
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A newly developed enzyme-linked immunosorbent assay (ELISA) for dehydroepiandros terone sulfate (DHEAS) and the chronological alterations of serum DHEAS levels in normal children are reported. The ELISA for DHEAS was developed by means of dehydroepiandrosterone (DHEA) -3-succinate conjugated to horseradish peroxidase and anti-DHEA-succinate-bovine serum albumin (BSA) serum. DHEAS was used as a standard. The sensitivity of the assay was 12.5 pg/well and the IC<SUB>50</SUB> was about 400 pg/well. The intra- and inter- assay coefficients of variation were <8% and <10%, respectively. There was close correlation between the DHEAS measurements by this ELISA method and the conventional radioimmunoassay (RIA) method (r=0.98, n=52). Serum DHEAS levels were determined in 923 normal children (485 males and 438 females) aged from 5 days to 18 years. The serum DHEAS levels were relatively high in newborns and decreased during the first year of life. The serum DHEAS levels stayed low until the age of 6 years, then increased exponentially until the age of 18 years. The serum DHEAS levels in males were significantly higher than in females at the 5-day and 13- to 18-year age groups (P<0.05), suggesting some testicular contribution to serum DHEAS levels in male subjects. In conclusion, this ELISA method for DHEAS is simple, sensitive, precise, accurate, and comparable to the conventional RIA method, and should provide a useful tool for the investigation of the physiological and pathological roles of adrenarche.
- 日本小児内分泌学会の論文
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