Development of New Evaluation Method for Whitening Agents by Using the Effect of .ALPHA.-MSH or Endothelin-1 on Normal Melanocytes.

概要

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It has been reported that α-MSH and Endothelin-1, which are secreted from keratinocytes or melanocytes by UV irradiation, are responsible for the pigmentation of skin according to the increasing of tyrosinase activity. To prevent or improve the pigmentation due to UV-irradiation. we notice the blocking of the actions induced by α-MSH or Endothelin-1. In this study, we established a convenient evaluation method for the blocking of the cytokine-induced reactions. The tyrosinase activity in normal human melanocytes was used as an index of the cytokine-induced reaction. With this method, it was found that certain plant extracts showed the blocking effect for increasing tyrosinase activity, Artemisia capillaris Thunberg and Sanguisorba officinalis L. for α-MSH and Achillea millefolium L., Carthamus tinctorius L., Eupatorium fortunei Turcz, Polygonum cuspidatum Sieb. and Thymus vulgaris L. for Endothelin-1. No alterations of these plants on tyrosinase activity in normal human melanocytes suggested that the blocking on the cytokine action contributed to their effects.
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