The Efficacy of Bovine Brain Extract on Human Fibroblast.
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概要
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In contemporary society which is composed largely of elderly people, "Aging" is a significant problem. Thus, research into anti-aging has become popular in various fields. In this context, cosmetic products are expected to prevent skin aging. Wrinkles are one of the typical symptoms of skin aging which is caused by functional weakening and structural change in the dermis. The activation of dermal cells is one of the major methods used against wrinkle formation.Recently, there has been a great deal of research done about the cellular activator which comes from animals or plants. In particular, the materials from the bovine are much used in cosmetic products. We have done research on bovine brain components for cosmetic products and found that the lipids contained in the bovine brain are effective at holding water in the horny layer of the skin.In this paper, we report the effect of the bovine brain extract on the fibroblast synthesizing the collagen and acid mucopolysaccharide, which are the major components of the dermis.The effect of the bovine brain extract was estimated with three methods; the cellular activation effect using the MTT-method, the collagen synthesis and the proliferation of the skin fibroblast. The results of these tests demonstrated that the bovine brain extract caused the activation of fibroblasts. A detailed analysis of the results revealed that the peptide fraction was effective on the collagen synthesis and the phospholipids on the proliferation of fibroblasts. The fraction containing phosphatidyl ethanolamine separated from phospholipids in particular has a great effect on cell growth.
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