Estrogen biosynthesis in human Fallopian tube.

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The activity of androstenedione aromatase (estrogen biosynthetase) was measured in Fallopian tubes from 31 women. Homogenates of the specimens (500 mg w. w. in 2ml of 1/15 M-bisodium phosphate buffer, pH7.4) were incubated with [19-3H, 4-14C]-androstenedione (3H, 1×106 dpm, 14C, 5×103dpm) and 1mg of NADPH at 37°C for 1 h in the air. Using Amberlite XAD-2 resin-charcoal column chromatography, we assayed the activity of aromatizing enzyme for androstenedione by the amount of 3H2O released. In 9 of the 20 pairs of Fallopian tubes from women in the reproductive age, aromatase activity was present; the range was 2 to 19 fmol/h/500 mg w. w., and the difference between the follicular and the luteal phases was not significant. In the specimens from 11 post menopausal women, aromatase activity was not detected (under 10 fmol/h/500 mg w. w.). Thus, there is some androstenedione aromatase activity in human Fallopian tubes, but the level of activity is significantly less than in the myometrium and endometrium. It should be physiologically clarified why the Fallopian tube has aromatase activity.
近畿産科婦人科学会の論文