Studies on Antigenicity of Human Trophoblast and its Regulatory Factors using Mixed Leucocyte Trophoblast Interaction Technique

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From the point of transplantation immunity a successful intrauterine graft of genetically alien tissue can never be explained without a peculiar mechanism in the mammalia.The possible reason about the puzzling mechanism has been discussed by Medawar, Billingham and others. But almost all remains still unresolved.One's attention might focus on the immunological antigenicity of the trophoblast when a pregnancy is discussed from the immunological aspect. So that, an immunogenicity of normal human trophoblast at early gestational stage was investigated by Mixed Leucocyte Trophoblast Interaction (MLTI) technique in vitro.The results were as follows;(1) The optimum conditions of MLTI were established.(2) The response index of MLTI using trypsinized trophoblast and autochthonous leucocyte was 4.9.(3) In the experiment with the addition of pregnant serum, the response index was decreased to 1.9.(4) The antigenicity of trophoblast was increased twice degree when treated with trypsin solution.From the above results, it was confirmed that human trophoblast has an antigenicity, of which liberation is depressed with masking substance covering trophoblastic cell surface and also with an inhibitory factor in pregnent serum in in situ.
近畿産科婦人科学会の論文