Experimental Production of Microencephaly:On 5-Azacytidine Induced Microencephaly in Hamster

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概要

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• 5-Azacytidine is an analogue of nucleic acid precursor, cytidine. This compound has been known to be incorporated into nuclear DNA of the proliferating cells, thus killing them. It has been also elucidated that this compound can pass through the placenta and is subsequently incorporated into proliferating cells in the embryo.<BR>Pregnant golden hamsters were given 6mg/kg of 5-azacytidine intraperitoneally on the 13 th and 14 th or on the 15 th and 16 th day of gestation. Six animals, thus treated, were sacrificed, 8, 12 and 24 hours after injection. Embryos were recovered and their brains were examined. Pathological changes were localized in the matrix layer, where active cell proliferation was taking place to produce the matrix cell itself, neuroblast and later glioblast and ependymal cells. The matrix layer had lost its normal structure and contained numerous degenerating cells and nuclear debris.<BR>Most embryos of the hamsters treated on the 13 th and 14 th day of pregnancy were resorpted. Some 31 offsprings were produced from five mothers which were treated on the 14 th and 15th day of gestation. All brains of the offsprings were examinded at 10, 15, 20, 30 and 40 days of age. Cerebral hemispheres were markedly reduced in size and subsequently exposed the corpora quadr igemina completely. The brain weight, , the length of the cerebrum and fissula longitudinalis centralis and the cerebral width were conspicuously smaller than those of the control. Histological examination by Golgi Cox stain revealed decrease of cortical depth and marked disarrangements of cytoarchitec ture in the cerebral cortex. All the offsprings did not show any abnormal behaviour nor convulsive movement until 40 days of age when the last group of animals were sacrificed

• 一般社団法人 日本小児神経学会の論文

一般社団法人 日本小児神経学会 | 論文

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