Protein Purification Using Immobilized Metal Affinity Porous Sheet
スポンサーリンク
概要
- 論文の詳細を見る
An immobilized metal affinity porous sheet was prepared by radiation-induced graft polymerization and subsequent chemical modifications. A 2-mm thick porous sheet, made of polyethylene, with a pore size and porosity of 1.3μm and 75%, respectively, was irradiated with electron beam. An epoxy-group-containing vinyl monomer was graft-polymerized onto the irradiated porous sheet, followed by the conversion of the epoxy group into an iminodiacetate group. The resultant chelating porous sheet was cut into disks 13 mm in diameter suitable for the packing into the cylindrical cartridge. Nickel ions were immobilized before the permeation of a histidine (His) -tagged fusion protein solution through the porous sheet. Purification of His-tagged fusion green fluorescent proteins was demonstrated in a permeation mode using the nickel-ion-immobilized metal affinity porous sheet.
- 日本イオン交換学会の論文
日本イオン交換学会 | 論文
- Acidic Property of Fe(III)-Taeniolite (Review: New development of irorganic ion exchanger)
- 第4回イオン交換国際会議(ICIE '07)報告
- イオン交換応用シリーズ(2)燃料電池とイオン交換
- 国際会議報告 International Workshop on Frontiers and Interfaces of Ion Exchangeに参加して
- 磁気泳動法による液滴界面への常磁性金属イオンの吸着平衡の測定