Highly Sensitive Probe for Gene Analysis by Electrochemical Approach.
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概要
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Electrochemically active DNA ligands, including ferrocenyl DNA oligonucleotides and ferrocenyl naphthalene bis(carboximide), are reviewed in connection with development of an electrochemical DNA sensor. Ferrocenyl oligonucleotides are used as an electrochemical probe DNA detectable by high performance liquid chromatography with the electrochemical detector (HPLC-ECD) system and they enable the target DNA analysis at femto mole level. Ferrocenyl oligonucelotides are useful also as a polymerase chain reaction (PCR) primer to realize quantitative PCR. Ferrocenyl naphthalene bis(carboximide) is a general DNA detecting ligand. When coupled with a probe DNA immobilized on an electrode, the target DNA from plasmid, chromosomal DNA, and total RNA extracted from several sources can be analyzed with very high sensitivity. Ferrocenyl naphthalene bis(carboximide) bound to the DNA duplex serves as a pseudo-polyferrocene array and this unique architecture leads to enhanced electron transfer between reduced glucose oxidase and the electrode. Unpaired base(s) present on the mismatched base(s) of the DNA duplex can be detected by observation of a decrease in the amount of ferrocenyl naphthalene bis(carboximide) bound to such a mismatched DNA duplex. Hybridized DNA on the DNA microarray was visualized electrochemically in the aqueous solution by the scanning electrochemical microscope (SECM) coupled with the ferrocenyl naphthalene bis(carboximide).
- 公益社団法人 日本化学会の論文
公益社団法人 日本化学会 | 論文
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