A Simple Fractionation Method for Determination of Phosphorus Components in Phytoplankton: Application to Natural Populations of Phytoplankton in Summer Surface Waters of Tokyo Bay

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A simple procedure for fractionation of phosphorus components in natural populations of phytoplankton has been developed. By a combination of a trichloroacetic acid (TCA) extraction, a hot dilute acid treatment, a charcoal treatment and an organic solvent extraction, cellular phosphorus was divided into orthophosphate, nucleotide phosphorus, sugar phosphates, acid-soluble polyphosphates, lipid phosphorus, nucleic acid phosphorus, acid-insoluble polyphosphates, and residual phosphorus. The recovery of phosphorus was 94±5%. This technique was appliedto natural populations of phytoplankton in summer surface waters of Tokyo Bay. Nucleic acid phosphorus and orthophosphate were most abundant, and accounted for 30 to 50% of total phytoplankton phosphorus. The nucleic acid phosphorus was proportional to the biomass of phytoplankton, irrespective of ambient nutrient concentrations. The orthophosphate showed the largest change in association with thechange in cellular phosphorus content as well as in ambient phosphate. The orthophosphate serves as a phosphorus reserve in the natural populations of phytoplankton in summer surface waters of Tokyo Bay where <I>Skeletonema costatum</I> wasdominant. Acid-insoluble polyphosphates tended to increase when ambient concentration of phosphate was high, but their amounts were almost one order of magnitude lower than that of orthophosphate.
日本海洋学会の論文