Ammonium Uptake Kinetics and Interactions between Nitrate and Ammonium Uptake in Chattonella antiqua

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Ammonium uptake kinetics and interactions between nitrate and ammonium uptakewere examined in Chattonella antiqua. After the addition of ammonium to the cultureof C. antiqua, the ammonium concentration decreased linearly with time. The ammoniumuptake rate as a function of ammonium concentration followed the Michaelis-Menten equation;the maximal uptake rate was 2.0 pmol cell-1hr-1 and the half saturation constant, 2.2μM.Although the ammonium uptake was not affected by nitrate, uptake of nitrate was rapidly (<<15 min) suppressed by ammonium and a 50% reduction in nitrate uptake was observed atan ammonium concentration of ca. 2μM.
日本海洋学会の論文