Growth Characteristics of <I>Chattonella antiqua</I>:Part 2. Effects of Nutrients on Growth
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概要
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Nutrient requirements of a red tide flagellate, <I>Chattonella antiqua</I>, were investigated in a laboratory culture experiment. Growth of <I>C. antiqua</I> was supported by nitrate and ammonium, and by urea to a limited extent, but not by glycine, alanine and glutamate. Orthophosphate served as a good phosphorus source but glycerophosphate did not. Fe<SUP>3+</SUP> (1 μM) fully promoted the flagellate's growth in the presence of 80 μM of EDTA. The addition of Mn<SUP>2+</SUP> (0-20μM), Zn<SUP>2+</SUP> (0-10μM) and Co<SUP>2+</SUP> (0-0.4 μM) did not show any effect. Among three vitamins tested, only B<SUB>12</SUB> (6 ng 1<SUP>-1</SUP>) served as a growth factor. Glucose, acetate and glycolate did not improve growth in the light nor did they support growth in darkness. The minimum cell quotas for nitrogen, phosphorus, iron and B<SUB>12</SUB> were estimated to be 11 pmoles cell<SUP>-1</SUP>, 1.0, -0.09 and 1.1fg cell<SUP>-1</SUP>, respectively.
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