Two simple methods of determination of total whey protein for diagnosis of the type of mastitis.
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概要
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Total whey protein was considered to be useful for the diagnosis of the type of Mastitis. Two simple methods were devised to determine total whey protein.<BR>Bromophenol blue (BPB) tube method (temporary name): Mix 0.5m<I>l</I> of 0.2M acetic acid buffer solution, pH4.0, and 0.5m<I>l</I> of milk Centrifuge at 2, 000rpm for 5minutes Isolate whey. Dilute whey 10-20times with acetic acid buffer solution. Mix 1m<I>l</I> of diluted whey and 4.0m<I>l</I> of BPB solution (dissolve 5mg of BPB with 100m<I>l</I> of 0.2M citrate-HCI buffer solutionpH3.0). Determine with spectrophotometer at wavelength of 610μm.<BR>BPB filter paper method (temporary name): Mix 0.2M acetic acid buffer solution, pH4.0, and an equal volume ofmilk. Add 5μ<I>l</I> of mixed solution to BPB filter paper (dissolve 100mg of BPB with 100m<I>l</I> of 1.0% citrate solution. Dip a piece of filter paper in the solution and dry). Compare the color and yellow ring size with the standard (made with bovine serum albumin).
- 公益社団法人 日本獣医師会の論文
公益社団法人 日本獣医師会 | 論文
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