Mid-Infrared Spectroscopic Quantification of Ionic Dissociative Metabolites Based on Three Spectral Extraction Methods

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Using FT-IR spectrometer and attenuated total reflection techniques, we provided three midinfrared spectral extraction methods of ionic dissociative metabolites by focusing our attention on the mixture of glucose 6-phosphate (G6P), fructose 6-phosphate (F6P) and Tris buffer, in which the phosphoglucose isomerase reaction was assumed. The extraction method 1 was based on the spectra for the mixtures, and considered the possible interaction between the metabolites. The method 2 used the spectra for the independent aqueous solutions. The method 3 was based on the spectra for the mixture, from which the spectra of the Tris buffers were subtracted, and might be applicable to the quantification for the system in which almost all the components except for the objective ones could not be specified. By using the spectra of the ionic species extracted by the three methods, we could accurately estimate the pH value and each reagent concentration in the G6P-F6P-Tris mixture.
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