Characteristics of Pulsed Electric Field Inactivation System Using Spiral Electrode Configuration

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Characteristics of a spiral electrode sterilizer by applying high voltage pulse were studied experimentally. The reactor was consisted of two spiral wires that were connected to pulse source and ground. The survival ratios of <I>Escherichia coli</I> K-12 were obtained with varying applied pulse peak voltage, capacitance of pulse forming capacitor, electric conductivity of suspension, reactor volume, and electrode material. The survival ratios were decreased with increasing pulse peak voltage and capacitance value, however, they were dependent on the input electrical energy calculated by applied voltage and pulse forming capacitor. When 300 J/mL of electrical energy was applied, 10<SUP>-5</SUP> of survival ratio could be achieved, independent of the applied voltage and pulse forming capacitor. Electrical conductivity of the liquid greatly affected to the survival ratio where the sufficient inactivation was achieved less than 4 mS/cm. Reactor volume and electrode connection of the reactor did not affect to the survival ratio.
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