Factors affecting fertilization in vitro of mouse eggs after removal of cumulus oophorus
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概要
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Cumulus oophorus was removed from freshly superovulated eggs of Jcl: ICR mature mice with hyaluronidases under various conditions and the cumulus-free eggs were inseminated with epididymal spermatozoa preincubated for 1.5-2h. Cumulus removal caused reduced fertilization rates to varying degrees depending on the experimental conditions. Relatively high fertilization rates were only attained either by dissolving the cumulus with very low concentrations of hyaluronidase (hyaluronate lyase) or by increasing the calcium concentration in the fertilization medium to 3.42mM. Possible involvement of cumulus factor (s) assisting fertilization is discussed.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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