Effect of Short-term Preservation of Ovaries at 38°C on the Development of Bovine Follicular Oocytes Matured and Fertilized in vitro
スポンサーリンク
概要
- 論文の詳細を見る
Effect of short-term preservation of ovaries on the development of bovine oocytes matured and fertilized in-vitro was investigated. Bovine follicular oocytes were collected from ovaries that had been preserved in physiological saline for 2.5, 5 and 8h at 38°C. Oocytes surrounded with a compact layer of cumulus cells were cultured for 20-24h, and then inseminated with frozen- thawed spermatozoa capacitated with heparin. At 72h after insemination, oocytes were separated from the cumulus cells but retained in the same culture dish and further cultured up to 8-12 days after insemination.<BR>The proportion of embryos cleaved were significantly higher with oocytes from ovaries preserved for 2.5 and 5h (66%, 266/388 and 62%, 194/313, respectively, ) than with those of ovaries preserved for 8h (21.9%, 42/194), (p<0.01). Similarly the proportion of embryos developed to blastocysts were significantly higher with embryos from ovaries preserved for 2.5 and 5h (19.9%, 51/256 and 24.7% 48/194, respectively, ) than with those of ovaries preserved for 8h (2.4%, 1/42), (P<0.05).<BR>Fresh (8) and frozen-thawed (7) blastocysts obtained from ovaries preserved for 5h were transferred nonsurgically to 4 (fresh) and 3 (frozen-thawed) recipients.Two of the 4 and one of the 3 recipients became pregnant. One live calf was born from a female received fresh embryos.<BR>These results indicate that oocytes from ovaries preserved for 5h at 38°C have the developmental capacity to become calves.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
- 単胚移植に関するガイドラインと海外の動向
- エピジェネティックなヒト着床不全への新規治療戦略
- 可溶性HLA-Gは体外受精卵選別のマーカーとなりうるか
- 種々の発育段階にあるマウス卵子の生存性に及ぼる浸透圧的収縮の影響
- 体外作出牛胚のバイオプシ---2分割後の生存性とPCRによる性判別