Effects of Gonadotrophins on the In vitro Maturation and Fertilization of Pig Oocytes

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This study was conducted to determine the effect of PMSG and/or hCG (each 15 IU/ml) added to TCM -199 supplemented with 10% FCS and 3mg/m1BSA on the maturation, fertilization and the incorporation of <SUP>3</SUP>H - methionine of pigoocytes. The follicular oocytes were cultured for 48hr with or without supplementationof PMSG, hCG, given each alone or in combination to maturation medium. Theaddition of PMSG with or without hCG to maturation medium was effective ininducting meiotic resumption in pig oocytes. Fertilization rate was significantlyimproved by addition of hCG alone or with PMSG, compared to the control. Fromthese results, it is suggested that the sperm penetration rate of oocyte was increasedby hCG added to the maturation medium. The proportion of polyspermic oocytes, however, was not different among the treatments. Incorporation of methionine intooocytes measured after maturation culture was increased by addition of PMSGand/or hCG as compared to the control. These results indicate that PMSG and/orhCG added to medium during maturation is related to the development of cytoplasmof pig oocytes.
日本哺乳動物卵子学会の論文