In vitro fertilization by microinjection of bovine sperm into bovine oocytes matured in vitro
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概要
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By microsurgery, bovine spermatozoon was injected into bovine oocytes matured in vitro, to elucidate a role of sperm components such as acrosome, neck, middle piece and tailplaying in fertilization and subsequent development. The injected spermatozoon wasselected from the pretreated sperm as follow: 1) washed sperm 2) preincubated sperm 3) sonicated sperm head 4) freeze-dried sperm head 5) preincubated sperm head. The spermsuccessfully injected were almost decondenced and 10.5-26.4 percent of them developedinto male pronucleus in egg cytoplasm. The results indicate that bovine gametes may beavailable for in vitro fertilization by microsurgery, e. g. microfertilization, in spiteof these pretreatment of sperm, and also this technique shall be useful for elucidationthe mechanism of the development in an interaction between oocytes and spermatozoa.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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