Loss of Fertilizability of Cumulus-free Mouse Oocytes upon Exposure to DMSO and its Prevention by Fetal Calf Serum

概要

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In vitro fertilization of cumulus-free mouse oocytes was studied in an effort to find the cause of reduced fertilizability in frozen-thawed oocytes. It was found that 1.5M DMSO in Hepes-buffered saline exerted deleterious effect on the fertilizability of mouse oocytes when added at room temperature for a period as short as 30 seconds. Oocytes retained the fertilizability after exposure to DMSO at 4°C, although the fertilization rate was significantly reduced. All oocytes were fertilized when zona pellucida was removed after treatment with DMSO. Oocytes frozen-thawed in 1.5M DMSO supplemented with 20% fetal calf serum or its high molecular weight (≥30, 000) fraction retained their fertilizing ability. These results suggest that the deleterious effect of DMSO is primarily due to its direct effect on zona pellucida and is prevented by high molecular weight serum component (s) other than albumin.
日本哺乳動物卵子学会の論文