Sperm Distribution and the Acrosome Reaction in Oviducts During In Vivo Fertilization in the Mongolian Gerbil

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In the present study, sperm distribution in oviducts and the status of their acrosome caps in the mogolian berbil after artificial insemination were examined. Adult females of Mongolian gerbil were superovulated with PMSG-hCG treatment. At 2 hr after hCG injection artificial insemination was performed by exposing the uteri of females anesthetized with sodium pentobarbital and then, 0.05 ml of a dense sperm suspension was injected into each uterus. At 0.5 hr after artificial insemination, motile spermatozoa were observed in the lower isthmus of oviducts. At 1 hr after the insemination, motile spermatozoa were seen in the upper isthmus and the first appearence of motile spermatozoa in the ampulla were observed 7 hr after the insemination. Motile spermatozoa recovered from the lower isthmus 3 hr after the insemination were not occurred the acrosome reaction. While, in motile spermatozoa recovered from the ampulla of oviducts after 11 hr of the insemination the acrosme reaction was observed.
日本哺乳動物卵子学会の論文