Chimaera rats from aggregated embryo
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概要
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Mammalian chimaeras are used for reserch on embryology and interaction of distinctive cells in genetic disorder. Though chimaeric mice have been produced on a large scale, there are a few reports concerning chimaeric rats. The present study was attempted to produce chimaeric rats by aggregation between Wistar and Hybrid (Wistar x Brown Norway) embryos. The results obtained are summarised as follow.<BR>1. Aggregated embryos cultured in modified medium (Dulbecco's PBS (-) with Whitten's nutrient saline) or Ham's F-12 with 5% fetal calf serum developed to blastocyst in higher proportion compared with modified medium with 10% bovine serum albumin.<BR>2. A total of 185 aggregated embryos was transfered and eighteen of them developed to chimaeric rats. Fifteen of the chimaeric rats grew up to adult.<BR>3. Sex ratio of chimaeric rats was 2: 1.<BR>4. All of the chimaeric rats had reproductive ability.
- 日本哺乳動物卵子学会の論文
日本哺乳動物卵子学会 | 論文
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