カイコ消化液における抗Streptococcus蛋白質のin vivo作用の解析

概要

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The in vivo action of anti-Streptococcus protein (ASP) contained in the digestive juice (DJ) of silkworm larvae, Bombyx mori, was studied. DJ tested was collected after dissection of larvae which had fed and of frozen larvae; anti-Streptococcus activity (ASA) in the fraction of saturated precipitates with 80% ammonium sulfate prepared from the DJ of larvae that had fed was stronger than that from starved larvae. ASA in the fraction of the DJ from the larvae that had fed was present of higher dilutions such as 500 and 600, but was absent of lower dilution such as 50 to 200 times. On the other hand, ASA in the fraction of the DJ from starved larvae was present of lower dilutions and was absent after freeze preservation for 120 days, whereas in the fraction of DJ from larvae which had fed, ASA withstood a longer preservation. In the G75-A fraction obtained through a Sephadex G-75 column from the ammonium sulfate precipitate, ASA was present and was inhibited by the addition of other fractions such as G75-B and G75-C, containing protein substances. Based on these results, it is considered that ASP may be associated with various protein substances and that denaturation is prevented or the activity is controlled by the other protein substances in the intestine.
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