Bacillus thuringiensis結晶性毒素の溶解に関与する家蚕消化液プロテア-ゼの分離・精製〔Bacillus thuringiensis結晶性毒素に関する研究-4-〕

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The toxic crystal obtained from Bacillus thuringiensis was solubilized by the digestive juice of the silkworm larvae. The solubilization activity of the enzyme in the silkworm digestive juice was assayed by estimating the absorbance at 280nm of toxic solution. An enzyme solubilizing toxic crystal in the digestive juice of silkworm larvae was isolated and purified 70-fold by methylalcohol treatment, gel filtration on Sephadex G-75 and column chromatography on DEAE-Sephadex A-50. The enzyme also showed the proteolytic activity against casein, and the solubilization activity was similar to the proteolytic activity in optimum pH, pH stability and thermal stability. These results suggested that the enzyme solubilizing toxic crystal in the silkworm digestive juice had also a protease-like activity.
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