家蚕卵の休眠過程におけるポリオ-ル,グリコ-ゲン量の変動に及ぼす低温の影響
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In the diapausing eggs kept continuously at 25°C, the sorbitol and glycerol content increased abruptly concomitant with the decrease in glycogen content at diapause initiation. The high level of these polyols became to decrease gradually from 120 days after oviposition, but the glycogen content remained at a low level. When transferred from 25°C to 5°C at 87 days after oviposition, the diapause eggs accumulating the high levels of these polyols showed the marked changes in carbohydrate content: an abrupt decrease in sorbitol and glycerol content with a delayed increase in glycogen. This cold-treatment caused to break diapase completely. When the eggs were kept at the programmed temperature as 1°C to -2.5°C, the polyols content in eggs at either 1°C or to -2.5°C was observed to decrease slowly compared to that at 5°C. The other hand, glycogen content increased accompanying a decrease in these polyol content. After the eggs kept continuously at -2.5°C had temporally exposed a high temperature (15°C) for 3 days at 310 days, they were incubatedat 1°C. The sorbitol and glycerol content in these eggs decreased rapidly correspond to the abrupt decrease of glycogen, although the level of polyols could be observed in the eggs having been kept at 1°C or -2.5°C.These results suggest that the interconversion between glycogen and sorbitol might be affected by the cold conditions of different temperatures. Futhermore, the role of polyols in the diapause eggs was disccused with preservation of embryo in cold conditions.
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