平衡密度遠心法によるNosema bombycisを含む数種微胞子虫胞子の精製
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概要
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The present study was made to establish the purification method of mature microsporidian spores by iso-density equilibrium technique using sucrose or Percoll (colloidal silica, Pharmacia). Although it was unsuccessful to isolate mature spores from any of immature spores, host tissue debrises, and bacteria by centrifugation using a 50-60% (W/W) sucrose gradient, the isolation and purification were done by centrifugation using buffered Percoll.After centrifugation of Percoll mixed with partially purified Nosema bombycis spores in Beckman Type 65 rotor, at 73, 000g for 30min., a band consisting of mature spores was exclusively obtained. The recovery rate of mature spores averaged over 90% and no damage to the spores in the course of purification was observed.
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