A Method for the Histochemical Demonstration of Cytochrome Oxidase

概要

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The Nadi reaction has been known as the histochemical technique for the demonstration of cytochrome oxidase. But this method has several shortcomings as following ; diffusion and crystallization of the pigment in the tissue sections, rapid fading and lipid solubility of the pigment, and difficulty for counterstaining of the nucleus due to the instability of the pigment by the procedure.<BR>Burstone roported his works in which he employed, for histochemical demonstration of cytochrome oxidase, many sorts of combinations of naphthols and amines, or amines alone, in addition, a series of non naphtholic compounds with reactive methylen groups.<BR>Independently of his works, we tried to obtain the stable red dye stuff which might be adequate for the counterstain. The 1-phenyl-3-methyl-5-pyrazolone and diethyl-p-phenylendiamine were employed in stead of alpha-naphthol and dimethyl-p-phenylenediamine. The employment was found to be useful for the demonstration of this enzyme and superior to the original Nadi method. The counterstaining of the nucleus using methylgreen gave good contrast.
日本組織細胞化学会の論文