Histochemical Studies of Oral Epithelium

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1. No alkaline phosphatase activity was observed in the oral epithelium of human and experimental animals under normal conditions, but the enzymatic activity of the capillary and inflammatory connective tissue was shown moderately or strongly. 2. Acid phosphatase was localized in the epithelium, especially, in the superficial layer, and in strong activity was observed in st. granulosum and st. corneum of oral epithelium. 3. Esterase reaction was observed throughout the whole layer of the oral epithelium except for the hornified layer. 4. The localization of β-glucuronidase, β-galactosidase and β-glucosidase was the same, the enzyme reaction being located in st. germinativum and st. granulosum by the post coupling method technique. 5. Aminopeptidase activity was presented in the st. basale and adjacent spinocellualr layer. 6. Succinic dehydrogenase distributed strongly in the cytoplasm of the basal cell and gradually decreased toward the superficial layer. On the other hand, TPN and DPN diaphorase activities were dominant throughout all layers of the oral epithelium except for the hornified layer.
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