Histochemical Differentiation of Non-Specific Phosphatases and ATP-Phosphatases with Special Reference to Acid ATP-Phosphatase

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Ac. ATP-ase was histochemically demonstrated by applying the Takamatsu-Gomori principle. In the tissue cells specific to the organ, the distribution pattern of ac. ATP-ase was similar to that of non-specific ac. P-ase and that of alk. ATP-ase to that of non-specific alk. P-ase with the exceptions of the hypophysis and islets of Langerhans. Ac. ATP-ase was found in the erythrocytes, media of vascular walls, muscular layers of intestinal walls and the cardiac and skeletal muscles. Non-specific P-ase and ATP-ase both acid and alkaline in paraffin sections showed different stability against heated water, ac. ATP-ase being more stable than nonspecific ac. P-ase and non-specific alk. P-ase more than alk. ATP-ase. Chlorpromazine or mepazine can be used routinely as the inhibitor of non-specific ac. P-ase and the activator of ac. ATP-ase. Dexamethasone inhibited ac. ATP-ase markedly and alk. ATP-ase moderately.
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