Induction of Ovarian Maturation and Development of Eggs, Larvae and Juveniles of the Puffer, Takifugu exascurus, Reared in the Laboratory.
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Ovarian maturation of the puffer Takifugu exascurus (Jordan et Snyder) was induced, and embry-onic, larval and juvenile development was observed. The brood fish were collected in Tassha Bay, Sado Island (38°05'N, 138°15'E), during the spawning season in 1986 which seemed to extend from late June to mid-July. To each female 3mg acetone-dried pituitary of <I>Hypophthalmichthys molitrix</I> was injected to induce ovarian maturation, which took place in about 77 hours at a water temperature of 19.5-21.0°. The eggs obtained by hormone injection were artificially fertilized with the milt from a collected male. The hatched larvae were fed successively on rotifers <I>Brachionus plicatilis</I>, Artemia nauplii and minced fish meat, and reared for a period of about one year.<BR>The eggs were spherical, 1.24±0.04mm in diameter, demersal and adhesive. The egg-membrane Was transparent and yolk was orange in color, containing a cluster of small oil-globules. The incubation period was about 160 hours at a water temperature of 18.5-21.0°. The newly-hatched larvae, measuring 2.9-3.1mm TL, had 8+15=23 myomeres. Absorption of the yolk was completed 3 days after hatching, by which time the larvae had attained 3.5-3.6mm TL. Larval finfolds disappeared and rudimentary dorsal, anal and caudal fins formed at 4.1-4.4mm TL, in 6 days after hatching. In 9-day old larva (5.4mm TL), fin ray rudiments appeared on the dosal, anal and caudal fins and spine-like scale formed on the belly. In 16-day old specimens, 9.1-10.2mm TL, the full complements of fin rays were completed on all the fins and the fish reached the juvenile stage. The growth of larvae and juveniles reared in 1986-1987 is expressed by the following equations, where y is total length (mm) and x is days after hatching.<BR>y<SUB>1</SUB>=2.9420·1.0639<SUP>x</SUP>0≤x≤19 (r=0.998)<BR>y<SUB>2</SUB>=4.0286·1.0464<SUP>x</SUP>19≤x≤33 (r=0.998)<BR>y<SUB>3</SUB>=9.8854·1.0180<SUP>x</SUP>33≤x≤72 (r=0.996)<BR>y<SUB>4</SUB>=20.1555·1.0080<SUP>x</SUP>72≤x≤115 (r=0.998)<BR>y<SUB>5</SUB>=28.0610·1.0049<SUP>x</SUP>115≤x≤202 (r=0.995)
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