Plasma Vitamin E and Lipoproteins Relationship in Thoroughbred Horse

概要

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To clarify the metabolism of vitamin E in Thoroughbred horses, the relation between vitamin E and lipoproteins in plasma was investigated. Lipoproteins were separated by density gradient ultracentrifugation and vitamin E was extracted into methanol from blood plasma collected from nine stallions. The contents of tocopherols and lipoprotein fractions in plasma were determined by high performance liquid chromatography (HPLC) with electrochemical detection. The concentration of vitamin E in each lipoprotein was the highest in high density lipoprotein (HDL), the middle in low densitylipoprotein (LDL) and the lowest in very low density lipoprotein (VLDL). The change of α-tocopher olconcentra-It is had a resemblance to the total lipids concentrations in each lipoprotein fraction. tions suggested that vitamin E combine with lipoproteins in the blood plasma of the stallions and the distribution of vitamin E depends on the concentrations of total lipids in each lipoprotein.
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