Application of High-Performance Liquid Chromatography to Norepinephrine and Epinephrine Determination in Horse Plasma

概要

論文の詳細を見る
An experiment was carried out to establish a determination method of catecholamines (CA), which are indicators of sympathomimetic activity, in horse plasma. A high-performance liquid chromatography with the trihydroxyindole reaction commonly used in the human case was introduced, but failed to measure CA in horse plasma precisely. Then a deproteinization under low temperature upon extraction of CA and an improvement of mobile phase were conducted for modification. Following the above modification, an excellent precision was obtained by this method. Furthermore, the mean and standard deviation of normal values of CA in 10 healthy Thoroughbred horses were 184.9±56.7pg/ml with norepinephrine and 119.4±44.9pg/ml with epinephrine. They were close to those obtained by other methods. Thus, this modification was evaluated to be very effective in determining CA in horse plasma.
日本ウマ科学会の論文