Analysis of Serum LDH Isoenzyme Using a Cellogel Membrane in Thoroughbred Racehorses
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概要
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To contribute to the enzymological diagnosis of equine disease, an estimation method was established for serum lactate dehydrogenase (LDH) isoenzyme. Gelatinized cellulose acetate (Cellogel) membrane was studied for its routine application to the serum LDH isoenzyme fractionation. LDH isoenzyme determination was performed by a modification of the method of Shioya et al. The estimation method for equine serum LDH isoenzyme was modified with regard to the optimal condition of the lithium lactate and NAD concentration in the coloring solution. It was found that the optimal concentration of lithium lactate was 0.167 mol/l and that of NAD 1.5 mmol/l. Each fraction of serum LDH isoenzyme was compared with the serum protein fraction. When densitometry was done, the modified method was advantageous, because the Cellogel membrane was free from interference between LDH1 and the albumin fraction. The normal value of LDH isoenzyme was determined in 45 healthy Thoroughbred racehorses. Mean and standard deviation were 15.5±2.8% for LDH1, 27.2±3.2 for LDH2, 37.4±2.2 for LDH3, 16.5±4.1 for LDH4, and 3.4±1.5 for LDH5. In the analysis of organ LDH isoenzyme, a sharp and characteristic isoenzyme pattern was obtained, with an extensive LDH1 distribution in erythrocytes and heart muscle, and with an extensive LDH5 distribution skeletal in muscle and liver. LDH isoenzyme could be separated sufficiently by this modified method with a Cellogel membrane. Besides, the estimation method mentioned in this report was rather simple and more convenient for routine application than the determination method of LDH isoenzyme by agar-gel electrophoresis.
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日本ウマ科学会 | 論文
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