Analysis of LPS-Pyrogen Induced Fever by Detection of Endotoxin in Rabbit's Blood Plasma
スポンサーリンク
概要
- 論文の詳細を見る
How does the LPS-pyrogen act on the nervous system was analyzed by the Limulus test (pyrogen test) for endotoxin in blood plasma. A cell lysate prepared from amoebocytes of Limulus polyphemus is an extremely sensitive indicator for the presence of endotoxin (LPS-pyrogen) . The state or grade of gelation reaction characteristic of a quantity of endotoxin in plasma is dependent on the concentration of clottable protein in the amoebocyte lysate and endotoxin in the assay mixture. In intact rabbits, gelation reaction for endotoxin in blood plasma during fever induced by LPS-pyrogen injected intravenously was gradually diminished in the time course, though higher temperature in rectum was still kept. And the simultaneous detection of endotoxin in the liquor in central nervous system, the result of the gelation reaction was almost negative. On the contrary, gelation reaction tested in liquor during high fever induced by the lateral ventricular as well as by the subdural administration of LPS-pyrogen was strong positive, but was almost negative in blood plasma. These results suggest that LPS-pyrogen has a strong effect directly on the central thermoregulatory mechanism if it is administered in central nervous system, but LPS-pyrogen itself does not pass through the blood brain barrier. The first and the second peak of the biphasic response to intravenous or intraventricular administration of LPS-pyrogen might be mediated by both exogenous and endogenous pyrogen which were discussed from the results in the present investigation.
- 日本生気象学会の論文
日本生気象学会 | 論文
- スギ花粉の放出と拡散過程の解明 (〔第41回日本生気象学会大会〕シンポジウム:植物とヒトとのかかわり)
- 高温環境における局所冷却時の生理応答--人体熱モデルによる解析と被験者実験
- 花粉症について (〔第41回日本生気象学会大会〕シンポジウム:植物とヒトとのかかわり)
- 農村地域における高齢者住宅の温熱と空気環境の実態
- ドイツにおける健康関連分野での森林利用に関する研究