Effect of Diesel Exhaust Gas Exposure on the Mutagenicity of Polynuclear Aromatic Hydrocarbons
スポンサーリンク
概要
- 論文の詳細を見る
Nitration behavior of polynuclear aromatic hydrocarbons (PAH) was investigated by mutagenicity and chemical analysis. PAHs was applied uniformly to a Teflon-coated glass fiber filter and/or to diesel particles which were collected on a Teflon-coated glass fiber filter. PAHs used were pyrene (Py), benzo (a) pyrene (Bap), chrysene (Chry), benzo (ghi) perylene (BghiP), benzo (b) fluoranthene (BbF) and perylene (Pery). These PAHs samples were exposed for 4 or 8 hours to a diluted exhaust gas which prepared by diluting original diesel exhaust from a heavy duty diesel engine with fresh air in the ratio 1: 10, and by eliminating particles in the diluted exhaust by filtration, successively. Mutagenic activity of the exposed sample was measured by the pie-incubation method using <I>Salmonella typhimurium</I> TA98 strain without S-9mix. Chemical analysis was carried out by a fused silica capillary column gas chromatography with a nitrogen-phosphorus detector.<BR>Five out of 6 PAHs tested produced direct mutagens by the exposure to diesel exhaust gas. BbF did not produced direct mutagen in the both tests, filter-PAH test and the diesel particle-PAH test. Mutagenic activity produced by the filter-PAH test was in the order of Py, BaP, Chry, Pery and BghiP samples. In the case of the diesel particle-PAH test, the direct mutagenic activity was in the order of Py, Pery, Bap, BghiP and Chry samples. The activity in the diesel particle-PAH test was higher than those in the filter-PAH test. In the both tests, mutagenic activity was increased with the increase of exposure time.<BR>Gas chromatographic analysis showed the formation of l-nitropyrene from the Py sample, 3 kinds of mononitroBaP including 6-nitroBaP from the Bap samples. A nitre derivative produced from peryrene was suggested to be 3-nitroperylene. The amounts of the nitre compounds were increased with the increase of the exposure time in the both tests, and the amounts of these compounds produced in the diesel particle-PAH test were higher than those in the filter-PAH test like the results in the mutagenic activity measurement.
- 社団法人 大気環境学会の論文
社団法人 大気環境学会 | 論文
- Spatio-Temporal Variations of Daily Average Concentration of NO2 in an Area with Trunk Roads
- Autoxidation of Methyl linoleate Initiated by Combustion Smoke of Combustibles
- Spatio-Temporal Variations of Daily Average of NO2Concentration in the Vicinity of a Crossroads
- Long-lived Gas-phase Radicals from Combustion
- Effective Combinations of Trapping Adsorbents and Extractive Solvents for Measuring Airborne Pesticides