Experimental Studies on the Mice Exposed to Sodium Nitrate and Sodium Nitrite Aerosols. I

概要

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The aerosol generation system was deviced for the studies on the exposure experiments of the mouse to nitrate and nitrite aerosols. Aerosols were generated with an ultrasonic nebulizer (Acoma EN-2), and passed into an 80 liter exposure chamber. Aerosol monitoring and characterization were performed by light scattering dust counter, cascade impactor, and electronmicroscope. 0.02M NaNO3 aqueous solution was used for aerosol generations. It was shown that concentrations of 15-89mg/m3 NaNO3 aerosols were achieved by adjusting dilution air flow. In the concentration range of 17 to 60mg/m3 and in the humidity range lower than the specific deliquescent humidity (NaNO3: 77.7%, NaNO2: 66%), log normal distributed aerosols with mass median aerodynamic diameter (MMAD) of 0.85-0.88μ and σg of 1.4-1.6 were obtained. Aerosols in the chamber distributed homogeneously. Four hours operation at the concentration of about 50mg/m3 showed that the coefficient of variation was 2.7% and MMAD and σg were constant. The aerosol flow rates of the system were of 11 to 49 litter/min.
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