Therapy for cutaneous leishmaniasis. The effect of temperature on the movement and multiplication of Leishmania promastigotes as a basis for local heat therapy.

概要

論文の詳細を見る
Leishmania was isolated from the skin of a Japanese man who had spent time in Saudi Arabia, and the movement and multiplication of promastigotes in NNN medium was observed after heating. 1) Promastigotes became immobile after 24 hr of heating at 56°C and 42°C, but they survived at 37°C, 25°C and 7°C. 2) When the level of water in the water bath at 42°C and of liquid of NNN medium in test tubes were the same, promastigotes lost their motility after continous heating for 4 hr and did not regain it until the 14th day at 25°C. 3) When the level of the water in the water bath at 42°C was 0.7 mm above the top of a solid slope of NNN medium in test tubes and 2.5 cm above the liquid surface (deeper water), promastigotes lost their motility after continous heating for 3 hr and did not regain it until the 14th day at 25°C. 4) Promastigotes lost their motility after heating 3 times for 1 hr at 42°C in the deeper water with intervals of cooling for 15 min at 25°C. 5) In the case when water levels were the same, promastigotes sometimes multiplied again, but this did not reoccur in the deeper water.
日本熱帯医学会の論文