Sensitivity of the biotin-avidin-peroxidase (BAP) technique for rabies virus neutralizing antibody assay and the measurement of neutralizing antibodies of vaccinated human sera.

概要

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The biotin-avidin-peroxidase (BAP) technique for measuring serum neutralizing (NT) antibody against rabies virus in human sera was established. When the focus reduction rates with serial serum dilutions were plotted on a probit chart, a linear regression line was drawn. The slopes (b-value) of the regression lines were identical irrespective of the different test samples. The sensitivity of the BAP test for detecting NT antibody was highest when compared with that of the rapid fluorescent focus inhibition test (RFFIT) and mouse neutralization test. In addition, low level-antibody titer (< 1 : 5) which could not be detected by mouse neutralization test was detected by the BAP test and the RFFIT. Serum NT antibody response w4s easily followed by the BAP test with individuals who received 3 doses of preexposure preventive rabies vaccine. Most of the vaccinees produced a detectable amount of serum NT antibody by a single dose of vaccine and after the second vaccination, all vaccinees produced high level of the antibody and the titers increased continuously until day 21, however, the titers on day 78, two months after the last vaccination, showed a tendency of decline.
日本熱帯医学会の論文