In Vitro Rearing of the Larval Endoparasitoid, Venturia canescens (GRAVENHORST) (Hymenoptera: Ichneumonidae). III. Growth Promoting Ability of Extract of Galleria mellonella Pupae.

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Pre-germband stage eggs of the larval endoparasitoid, Venturia canescens, developed up to the pupal stage in an insect cell culture medium, MGM-450, mixed with an equal amount of pupal extract of the host, Galleria mellonella. Among the pupal extracts of various stages tested, that from 1 day old pupae was most effective, and supported development of approximately 95% of the eggs to the last stadium. The matured larvae successfully pupated if rearing wells were plugged with cotton. One of the pupae obtained reached the pharate adult stage, but failed to ecdyse to an adult. Larval development was retarded when the concentration of pupal extract was reduced, and no larvae on a 3.1% pupal extract larvae ecdysed to the 2nd stadium. However, larvae which showed arrested growth in such medium developed to the last stadium after the medium was changed to the one containing 50% pupal extract. The growth-promoting ability of the pupal extract was lost by heat-treatment at 80°C for 10 min or by passage through a molecular sieve of 100, 000 M.W., suggesting that the growth-promoting factor(s) in the host pupal extract was protein.
日本応用動物昆虫学会の論文