Mass-rearing of the onion and seed-corn flies, Hylemya antiqua and H. platura (Diptera: Anthomyiidae), on an artificial diet with antibiotics.

概要

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A simple and inexpensive artificial diet was developed for mass-rearing the onion fly, Hylemya antiqua MEIGEN and the seed-corn fly, H. platura M. The diet consists of a commercial food for guinea pigs, defatted soybean flour, dehydrated yeast, sugar, agar, micro-nutrients and antibiotics. A large amount of cellulose powder was also included to improve the physical quality of the diet. The advantages of this diet are : 1) no sterilization of egg surface or disinfection of the rearing container prior to inoculation is required; 2) growth of the larvae is fairly good and uniform; 3) yield is considerably high (over 65%) and pupal size is comparable to those reared on onions; 4) the rearing condition is quite hygienic and the diet discharges no unpleasant odor as do onion and other artificial diets. Screening of several antibiotics showed that kanamycin sulfate singly, and the mixtures of neomycin-chloramphenicol and spiramycin-chloramphenicol effectively suppress the frequently observed larval diseases. A neomycin-chloramphenicol mixture was used in the standard diet since these are less expensive than the other antibiotics.
日本応用動物昆虫学会の論文