Morphological Changes with the Passage of Time of Fibroblasts After Soft Laser Irradiation
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To elucidate empirical findings about the accelerated wound healing after soft laser irradiation, we examined how soft laser rays activated mouse normal fidroblasts with varying exposure conditions. Then, we observed interesting morphogical changes in the fibroblasts under the optimal exposure conditions.<BR>We used a gallium-arsenide diode soft laser system with the mean output of 2mW emmiting a beam of 904nm wavelength. The cells tested were fibroblasts from mice. We evaluated various exposure conditions on the basis of the growth of mitochondria defined by MTT colorimetry 24 hours after exposure. Next, cellular exposure was made with the optimal exposure conditions, and compared cells with and without exposure to the soft rays immediately, 5, 10, 15 and 18 hours after exposure for their morphologic changes.<BR>The optimal condition selected for the mouse fibroblasts was 4-minute A mode irradiation repeated three times. The morphologic exathination showed that those fibroblasts were originally spindle-shaped immediately after cell division in both groups. Five hours later the fibroblasts without exposure to the soft ray had changed from the spindle-shaped to polygons, while the exposed ones were spindle-shaped with long cytoplasmic filaments. Ten hours later the not exposed cells had changed to big polygonal bodies, while the exposed cells had changed to the spindle shape with several cytoplasmic filaments. Fifteen hours the not exposed cells remained the same polygonal shapes as before, while the exposed cells almost began to devide. Eighteen hours later the not exposed cells had changed in shape from polygonal to spindle-shaped, while the exposed cells had completed the cell division to be spindle-shaped with long cytoplasmic filaments. In summary, it was clearly demon strated that the soft laser rays directly act on the fibroblasts to accelerate their cell growth.<BR>(J. Jpn. Soc. Laser Dent. 7: 1-5, 1996 Reprint requests to Dr. Takagi)
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