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CO2 laser is known to stimulate reparative dentin formation by odontoblasts, but the precise mechanism and molecular basis of this effect remain unclear. This study was designed to elucidate the effect of CO2 laser on calcified nodule formation in mouse odontoblasts. MDPC-23 (mouse odontoblast-like cells) were serum-starved for 24 hours and irradiated by a CO2 laser apparatus at 2-5 W, and then incubated with a mineral-inducing medium containing ascorbic acid and β-glycerolphosphate. Laser irradiation at 2 W for 30 sec had little effect on cell proliferation examined by cell counting kit-8. ALP activities in irradiated cells on day 2 and 4 were significantly higher than in the control cells. However, mRNA expressions of bothdentin sialophosphoprotein (DSPP) and type I collagen were not affected by laser irradiation. The total number and areasof calcified nodules in irradiated cells were apparently increased compared with those in control cells. The secretion of collagen into the medium measured by the Sirius red staining method was significantly accelerated by irradiation on day 1 and 2. Interestingly, irradiation stimulated both mRNA and protein expressions of HSP47 (collagen specific chaperone) within 24hours. These results suggest that CO2 laser irradiation enhanced calcified nodule formation in mouse odontoblast-like cells byinducing the expression of HSP47 which is essential for collagen production.
日本レーザー歯学会の論文