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Recently, application of low-power laser rays to wound healing of oral soft tissue has becomeroutine. To elucidate the effects of a low-power laser on wound healing at the oral mucosa, we examined thechange of cytoskeletal formation of normal human gingival fibroblasts after irradiation of low-power laserrays <I>in vitro</I>, by confocal laser scanning microscopy.<BR>Cells were cultured for 4, 7, and 24 hours after laser irradiation once, for 30 sec, with a diode laser (905nm, GaAlAs diode, pulse wave, 1.2kHz, 10W (peek), 2.4mW (average), 1.25J/cm<SUP>2</SUP> (150sec)), and cells cultured forthe same times without laser irradiation were used as control groups.<BR>After 4 and 7 hours, developed actin filaments were observed, associated with increasing and accumulatingfibronectin in extracellular component on the basal surface of fibroblasts, in the laser irradiation groups.However, actin filament and fibronectin were not significantly different compared with the control groups after24 hours.<BR>These results suggest that low-power laser irradiation activates cell adhesion ability and promotes thedevelopment of actin filament and early accumulation of fibronectin of human gingival fibroblasts.
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