p53 Mutations in Non-small Cell Lung Cancers.

概要

論文の詳細を見る
Mutations of the p53 gene are one of the most frequent genetic abnormalities in lung cancer. However, the relationship with metastatic spread in lymph nodes is still unclear. We investigated 47 specimens of primary non-small cell lung cancer and 8 specimens of metastatic lymph nodes, using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis and sequencing on exon5 to exon9 of the p53 gene. In the primary tumors, 12 mutations (25.5%) were identified. Three samples presented G to T transversion, reflecting the influence of benzopyrene contained in tobacco smoke, whereas no significant correlations were found between p53 mutations and smoking indices. In lymph node metastases, 2 mutations were found, which were identical with those in the primary tumors. In these cases, both aberrant and normal SSCP bands were observed. Quantitative analysis of aberrant and normal bands revealed that there was no significant difference in aberrant-normal p53 ratio between the primary tissue and metastatic lymphnodes. Our results suggest that there is no correlation between p53 mutation and lymph node metastasis in non-small cell lung cancer.
特定非営利活動法人日本肺癌学会の論文