The Establishment of a Cell-Line (DL-1) from Experimental Mongrel Lung Cancer and The Results of Transplantation of The Cultured Cells to Allogeneic Mongrels

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A cell line (DL-1) from a mongrel lung cancer, induced experimentally byintrabronchial application of 20-methylcholanthrene was established in vitro andmaintained in continuous growth for about two years. The materials used forexplantation was a metastatic adenoacanthomatic paratracheal lymph node. The cultured cells grew attached to a glass surface in layered heaps and posessedthe characteristics of poorly differentiated cancer cells from their ultrastructure anda tumor which grew in a hamster's cheek pouch after transplantion of 15th generationcells. The cultured cells were transplanted to 15 allogeneic mongrels using manydifferent methods and amounts of cells. But we could not succeed in transplantingthe cultured cells.
特定非営利活動法人日本肺癌学会の論文