Specific Cell Surface Antigen in Human Pulmonary Carcinoma of Oat Cell Type Detected by Membrane Immunofluorescence

概要

論文の詳細を見る
Search for possible tumor-specific reaction in human lung cancer was attempted by the indirect membraneimmunofluorescence technique. Living cells of the established cell line, OAT, derived from human pulmonarycarcinoma of oat cell type, were used as the target cells and both FITC-conjugated anti-human IgG and Ig Mgoat sera were used as the second reagents, respectively. Five out of 8 sera from the patients with pulmonarycarcinoma of small cell type gave positive membrane immunofluorescence reaction with anti-Ig M reagent but notwith anti-IgG. However, 27 sera from patients with other types of carcinomas, including 9 cases of adenocarcinomaof the lung, and 8 sera from healthy individuals gave no positive reaction. No membrane immunofluorescencereaction was given by the selected positive sera, when it was tested against the cell line of Burkitt lymphoma origin, P3HR-1. The results strongly suggest that the antibody against the cell surface of OAT cells resides in IgM fractionof sera from the patients with small cell carcinoma and that the antigen specific for and common to small cellcarcinomas of the lung.
特定非営利活動法人日本肺癌学会の論文